Traditional nucleic acid extraction, isolation and purification procedures are labor intensive, require multiple pieces of laboratory equipment and are not specific for nucleic acids. The standard practice utilizes ionic charge as a means for interaction of nucleic acids to either microsphere beads or filters in spin columns. This interaction is not specific for nucleic acids, the location or orientation of the nucleic acid molecules. Our solution provides for nucleic acid specific binding in a single 3’ orientation. These properties allow for direct affinity or covalent binding which allows the nucleic acid molecule to be used directly in multiple molecular biology applications, such as cDNA synthesis, primer extension, regeneration of original input material and PCR based methodologies.
Assays performed on the bound nucleic acid molecules will produce materials suitable for PCR and NGS based analysis. With SimplSeq’s process, NGS assays will not require ligation, multiple PCR stages or complicated targeted capture hybridization steps.
The completed NGS library will be processed in fewer steps, with far less manipulation providing a more robust system without the traditional methods that contribute to known errors and shortcomings in the standard NGS sample preparation assays.